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Image Search Results
Journal:
Article Title: Actinomyces naeslundii Displays Variant fimP and fimA Fimbrial Subunit Genes Corresponding to Different Types of Acidic Proline-Rich Protein and ?-Linked Galactosamine Binding Specificity
doi:
Figure Lengend Snippet: Hybridization of full-length and central fimP (type 1) and fimA (type 2) gene probes with chromosomal DNA from an independent reference collection of Actinomyces strains and with DNA from a single strain characterized by a statherin binding pattern
Article Snippet: 2:1 2:2
Techniques: Hybridization, Binding Assay
Journal:
Article Title: Actinomyces naeslundii Displays Variant fimP and fimA Fimbrial Subunit Genes Corresponding to Different Types of Acidic Proline-Rich Protein and ?-Linked Galactosamine Binding Specificity
doi:
Figure Lengend Snippet: The type 1 and type 2 fimbrial subunits are encoded by single gene copies in Actinomyces isolates. Southern blot hybridization of chromosomal DNA from A. naeslundii isolates with the fimP type 1 central DNA probe (left panel: lane 1, strain B-1-K; lane 2, P-1-K; lane 3, T14V; and lane 4, LY7), the fimA type 2:1 central DNA probe (middle panel: lane 1, strain Pn-22-E; lane 2, Pn-6-GA; lane 3, P-5-N; and lane 4, ATCC 12104T), and the fimA type 2:2 central DNA probe (right panel: lane 1, strain P-1-K; lane 2, P-1-N; and lane 3, LY7). Chromosomal DNA was digested with BamHI prior to hybridization with either type 1 or type 2:1 DNA probes and with PstI prior to hybridization with the type 2:2 DNA probe. The sizes (in kilobases) of HindIII-digested bacteriophage lambda DNA standards (lanes kb) are indicated on the right.
Article Snippet: 2:1 2:2
Techniques: Southern Blot, Hybridization, Lambda DNA Preparation
Journal:
Article Title: Actinomyces naeslundii Displays Variant fimP and fimA Fimbrial Subunit Genes Corresponding to Different Types of Acidic Proline-Rich Protein and ?-Linked Galactosamine Binding Specificity
doi:
Figure Lengend Snippet: Alignment of the deduced amino acid sequences (in single-letter code) of central segments of the fimP and fimA type 2:1 and type 2:2 fimbrial subunit genes (see Fig. Fig.1).1). Identical amino acids are indicated by gray shading, and those conserved in structure are indicated by a colon (:). Amino acids in boldface type are variable in fimbrial subunit sequences corresponding to the same binding specificity. The type 1 sequence data were collected from five isolates of genospecies 2 (strains B-10-K, B-1-K, P-1-K, P-3-K, and LY7), the type 2:1 sequence data are from six strains of genospecies 1 (strains P-3-N, P-5-N, Pn-1-GA, Pn-6-N, Pn-20-E, and Pn-22-E), and the type 2:2 sequence data are from a comparison of three strains of genospecies 2 (strains P-1-N, P-1-K, and LY7).
Article Snippet: 2:1 2:2
Techniques: Binding Assay, Sequencing